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Produce a Dichotomus/Bacterial Key including the following characteristics

Instructions: Produce a Dichotomus/Bacterial Key including the following characteristics for the 12 GI Tract Pathogens as listed below. Base the key upon Gram positive or negative, Morphology, culturing and biochemical tests (glucose fermentation, coagulase + or -, catalse + or -,etc…).

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ORGANISM CHARACTERISTIC
Campylobaacter jejuni
Campylobacter jejuni is a slender, spirally curved, motile Gram-negative rod. s requirement for reduced levels of oxygen

utilizes a relatively large supply of serine, proline, aspartate, and glutamate;

Salmonella typhimurin Salmonella typhimurium is a Gram-negative, rod-shaped bacterium

g lactose negative, citrate positive, lysine decarboxylase positive, gelatin hydrolysis negative, and the production of hydrogen sulfide

Escherichia coli Gram-negative, rod-shaped bacterium that causes.

motilefacultative anaerobes (they can makes ATP energy by aerobic respiration if oxygen is present, but is also capable of switching to fermentation)

Clostridium perfringens Clostridium perfringens is a Gram-positive, spore-forming, heat-resistant, rod-shaped anaerobic bacterium, widely distributed in soil, marine sediment, decaying vegetation, and the intestinal tract of insects, humans and other vertebrates. The toxin is a zinc metallophospholipase that requires zinc for activation.
C. dificile Endospore-Forming Gram positive rods and cocci

oxygen requirements, motility, morph, catalase

Alcaligenes faecalis

. It was originally named for its first discovery in feces, but was later found to be much more common in other places as well. Optimum growth occurs at about 37°C with no pigmentation. Whileopportunistic infections do occur, the bacterium is generally considered non-pathogenic. When an opportunistic infection does occur, it is usually observed in the form of a urinary tract infection

Gram-negative, rod-shaped, motile, non-nitrate reducing, oxidase positive, catalase positive, alpha hemolytic, and citrate positive(variable)obligate aerobe that is commonly found in the environment
Enterobacter faecalis Facultatively Anaerobic Gram negative rods

growth factors, morph., gram rxn., oxidase rxn., read Genera descriptions

Shigella sonnei

1).Shigella are closely related toEscherichia coli andSalmonellaand share common antigens with one another and other enteric bacteria. There are four species ofShigella:S. boydii, S. dysenteriae, S. flexneri,?and S. sonnei.

Shigella is a genus of bacteria belonging to the family Enterobacteriaceae. Species of this genus are Gram-negative, rod-shaped, and non-lactose fermenting (with exception toS. sonnei). They are also non-motile due to the lack of flagella and makes ATP (an energy molecule) by aerobic respiration if oxygen is present, but is also capable of switching to fermentation –facultative anaerobe.Shigella species can ferment carbohydrates with the production of acid, and grow in translucent white colonies (Figure
Bacillus cereus

Some strains are harmful to humans and causefoodborne illness, while other strains can be beneficial as probiotics for animals.[1] It is the cause of “Fried Rice Syndrome,” as the bacteria is classically contracted from fried rice dishes that have been sitting at room temperature for hours (such as at a buffet).[2

Bacillus cereus is an endemic, soil-dwelling, Gram-positive, rod-shaped, beta hemolytic bacterium.
Vibrio cholerae Facultatively Anaerobic Gram negative rods

growth factors, morph., gram rxn., oxidase rxn., read Genera descriptions

Helicobacter pylori

(Figure 1). The species name stems from:Helico, meaning helical or spiral shape,bacter, meaning bacteria, andpylori, refering to pylorus region of the stomach. This pathogen possesses five to seven distinct flagella and grows best when cultured at 37°C (Figure 2). Interestingly, in resting individuals, 37°C is the stomach’s temperature – the targeted organ; however, the bacteria is able to withstand temperatures as low as 25°C.

Helicobacter pylori is a Gram-negative, curved S-shaped bacterium, which also has a non-cultural coccoid form with rounded ends
Listeria monocytogenes
Regular, Nonsporlating Gram positive rods

morph., oxygen require, catalase

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